On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood

On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood

The extraction of virological markers in white blood cells (WBCs) from whole blood--without reagents, electricity, or instruments--is the most important first step for diagnostic testing of infectious diseases in resource-limited settings. Here we develop an integrated microfluidic chip that continuously separates WBCs from whole blood and mechanically ruptures them to extract intracellular pro...

One-step White Blood Cell Separation from Whole Blood On a Centrifugal Microfluidic Device

In this work, we propose a fully automated WBCs isolation device from biological sample utilizing centrifugal microfluidics on a polymer based CD platform. Using the novel Laser Irradiated Ferrowax Microvalves (LIFM) and liquid density gradient medium on CD platform, the total process of a blood sample loading on the density gradient medium, fractionating to concentrate the WBC, decanting plasm...

White Blood Cells Identification and Counting from Microscopic Blood Image

The counting and analysis of blood cells allows the evaluation and diagnosis of a vast number of diseases. In particular, the analysis of white blood cells (WBCs) is a topic of great interest to hematologists. Nowadays the morphological analysis of blood cells is performed manually by skilled operators. This involves numerous drawbacks, such as slowness of the analysis and a nonstandard accurac...

White Blood Cells Identication and Classication from Leukemic Blood Image

The counting and classification of blood cells allows the evaluation and diagnosis of a vast number of diseases. Through the analysis of white blood cells (WBCs) the ALL Acute Lymphocytic Leukemia, a blood cancer that can be fatal if left untreated, can be detected. Nowadays the morphological analysis of blood cells is performed manually by skilled operators. This involves numerous drawbacks, s...

Completely automated extraction of DNA from whole blood.